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Publication

Property	Value
Working Groups	RG Köster, RG Moser, RG Outeiro, RG Pape, RG Preobraschenski, RG Rizzoli
Subproject	None
Open Access	Yes
Publication Type	Journal Article
Peer Reviewed	Yes
DOI	10.1038/s41587-024-02431-9
Publication Year	2024
Title	One-step nanoscale expansion microscopy reveals individual protein shapes
Journal	Nature Biotechnology
ISSN	1087-0156
eISSN	1546-1696
URL	https://www.nature.com/articles/s41587-024-02431-9
Journal Abbreviation	Nat Biotechnol
Extra	RTG 2824
Authors	Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal KA, Alawieh MM, Alawar N, Eilts J, Kang J, Soleimani A, Müller M, Pape C, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO
First Author	Shaib AH
Last Author	Rizzoli SO

External Resources

nature.c...-9.pdfhttps://www.nature.com/articles/s41587-024-02431-9.pdf Article fulltext

gro-2/145891http://resolver.sub.uni-goettingen.de/purl?gro-2/145891 GRO.publications identifier

9606https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 NCBI taxonomy (human, Homo sapiens)

10116https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 NCBI taxonomy (rat, Rattus norvegicus)

0009-0007-2656-3820https://orcid.org/0009-0007-2656-3820 ORCID identifier (Ali H. Shaib)

0009-0009-6973-1630https://orcid.org/0009-0009-6973-1630 ORCID identifier (Abed Alrahman Chouaib)

0000-0002-6717-4721https://orcid.org/0000-0002-6717-4721 ORCID identifier (Rajdeep Chowdhury)

0009-0003-2515-8330https://orcid.org/0009-0003-2515-8330 ORCID identifier (Jonas Altendorf)

0000-0002-8375-3360https://orcid.org/0000-0002-8375-3360 ORCID identifier (Daniel Mihaylov)

0009-0003-1151-7726https://orcid.org/0009-0003-1151-7726 ORCID identifier (Donatus Krah)

0000-0002-4814-9010https://orcid.org/0000-0002-4814-9010 ORCID identifier (Russell K. W. Spencer)

0000-0001-7978-8811https://orcid.org/0000-0001-7978-8811 ORCID identifier (Svilen Veselinov Georgiev)

0009-0007-5712-7175https://orcid.org/0009-0007-5712-7175 ORCID identifier (Nikolaos Mougios)

0000-0003-4847-4144https://orcid.org/0000-0003-4847-4144 ORCID identifier (Sofiia Reshetniak)

0000-0001-8117-1431https://orcid.org/0000-0001-8117-1431 ORCID identifier (Tiago Mimoso)

0009-0003-1357-5161https://orcid.org/0009-0003-1357-5161 ORCID identifier (Han Chen)

0000-0002-4637-1873https://orcid.org/0000-0002-4637-1873 ORCID identifier (Parisa Fatehbasharzad)

0000-0001-9706-3734https://orcid.org/0000-0001-9706-3734 ORCID identifier (Kim-Ann Saal)

0000-0002-8591-2513https://orcid.org/0000-0002-8591-2513 ORCID identifier (Nadia Alawar)

0000-0002-7472-973Xhttps://orcid.org/0000-0002-7472-973X ORCID identifier (Marcus Müller)

0000-0001-6562-7187https://orcid.org/0000-0001-6562-7187 ORCID identifier (Constantin Pape)

0000-0003-1316-1906https://orcid.org/0000-0003-1316-1906 ORCID identifier (Luis Alvarez)

0000-0001-6407-1199https://orcid.org/0000-0001-6407-1199 ORCID identifier (Claudia Trenkwalder)

0000-0001-8437-3645https://orcid.org/0000-0001-8437-3645 ORCID identifier (Brit Mollenhauer)

0000-0003-1679-1727https://orcid.org/0000-0003-1679-1727 ORCID identifier (Tiago Fleming Outeiro)

0000-0002-1825-0433https://orcid.org/0000-0002-1825-0433 ORCID identifier (Julia Preobraschenski)

0000-0001-6005-7517https://orcid.org/0000-0001-6005-7517 ORCID identifier (Ute Becherer)

0000-0001-7145-0533https://orcid.org/0000-0001-7145-0533 ORCID identifier (Tobias Moser)

0000-0002-0419-3351https://orcid.org/0000-0002-0419-3351 ORCID identifier (Edward S. Boyden)

0000-0003-3783-1388https://orcid.org/0000-0003-3783-1388 ORCID identifier (Alexandru Radu Aricescu)

0000-0002-1692-3219https://orcid.org/0000-0002-1692-3219 ORCID identifier (Markus Sauer)

0000-0002-4968-9713https://orcid.org/0000-0002-4968-9713 ORCID identifier (Felipe Opazo)

0000-0002-1667-7839https://orcid.org/0000-0002-1667-7839 ORCID identifier (Silvio-Olivier Rizzoli)

255https://sfb1190.med.uni-goettingen.de/production/literature/publications/255 Published Data Registry entry (CRC 1190)

295https://sfb1286.uni-goettingen.de/literature/publications/295 Published Data Registry entry (CRC 1286)

583https://prod.mbexc.uni-goettingen.de/literature/publications/583 Published Data Registry entry (MBExC, preprint)

1/517https://mbexc.uni-goettingen.de/archive/1/517 Research Data Archive (supplemental material)

043j0f473https://ror.org/043j0f473 ROR identifier (043j0f473, German Center for Neurodegenerative Diseases)

042nb2s44https://ror.org/042nb2s44 ROR identifier (042nb2s44, Massachusetts Institute of Technology)

03av75f26https://ror.org/03av75f26 ROR identifier (03av75f26, Max Planck Institute for Multidisciplinary Sciences)

00tw3jy02https://ror.org/00tw3jy02 ROR identifier (00tw3jy02, MRC Laboratory of Molecular Biology)

05xy1nn52https://ror.org/05xy1nn52 ROR identifier (05xy1nn52, Multiscale Bioimaging)

01kj2bm70https://ror.org/01kj2bm70 ROR identifier (01kj2bm70, Newcastle University)

0270sxy44https://ror.org/0270sxy44 ROR identifier (0270sxy44, Paracelsus Elena Klinik Kassel)

01jdpyv68https://ror.org/01jdpyv68 ROR identifier (01jdpyv68, Saarland University)

021ft0n22https://ror.org/021ft0n22 ROR identifier (021ft0n22, University Medical Center Göttingen)

01y9bpm73https://ror.org/01y9bpm73 ROR identifier (01y9bpm73, University of Göttingen)

00fbnyb24https://ror.org/00fbnyb24 ROR identifier (00fbnyb24, University of Würzburg)

AB_887857https://scicrunch.org/resolver/AB_887857 SciCrunch identifier (RRID:AB_887857)

AB_2210057https://scicrunch.org/resolver/AB_2210057 SciCrunch identifier (RRID:AB_2210057)

AB_2619811https://scicrunch.org/resolver/AB_2619811 SciCrunch identifier (RRID:AB_2619811)

CVCL_0063https://scicrunch.org/resolver/CVCL_0063 SciCrunch identifier (RRID:CVCL_0063, HEK293T)

CVCL_0042https://scicrunch.org/resolver/CVCL_0042 SciCrunch identifier (RRID:CVCL_0042, U2OS)

SCR_023662https://scicrunch.org/resolver/SCR_023662 SciCrunch identifier (RRID:SCR_023662, AlphaFold Protein Structure Database)

SCR_002285https://scicrunch.org/resolver/SCR_002285 SciCrunch identifier (RRID:SCR_002285, Fiji)

SCR_002798https://scicrunch.org/resolver/SCR_002798 SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)

SCR_018863https://scicrunch.org/resolver/SCR_018863 SciCrunch identifier (RRID:SCR_018863, Imspector)

SCR_013673https://scicrunch.org/resolver/SCR_013673 SciCrunch identifier (RRID:SCR_013673, Leica Application Suite X)

SCR_020233https://scicrunch.org/resolver/SCR_020233 SciCrunch identifier (RRID:SCR_020233, Leica TCS SP5 Broadband Confocal Laser Scanning Microscope)

SCR_018169https://scicrunch.org/resolver/SCR_018169 SciCrunch identifier (RRID:SCR_018169, Leica SP8 LIGHTNING confocal microscope)

SCR_001622https://scicrunch.org/resolver/SCR_001622 SciCrunch identifier (RRID:SCR_001622, MATLAB)

SCR_016137https://scicrunch.org/resolver/SCR_016137 SciCrunch identifier (RRID:SCR_016137, Microsoft Excel)

SCR_014551https://scicrunch.org/resolver/SCR_014551 SciCrunch identifier (RRID:SCR_014551, Olympus cellSens Software)

SCR_003210https://scicrunch.org/resolver/SCR_003210 SciCrunch identifier (RRID:SCR_003210, SigmaPlot)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41587-024-02431-9/MediaObjects/41587_2024_2431_MOESM1_ESM.pdf Supplemental material

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41587-024-02431-9/MediaObjects/41587_2024_2431_MOESM4_ESM.pdf Supplemental material (figure)

goeseek.gwdguser.de/studies/204https://goeseek.gwdguser.de/studies/204 Supplemental material (goeseek study)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41587-024-02431-9/MediaObjects/41587_2024_2431_MOESM2_ESM.pdf Supplemental material (reporting summary)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41587-024-02431-9/MediaObjects/41587_2024_2431_MOESM5_ESM.xlsx Supplemental material (source data)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41587-024-02431-9/MediaObjects/41587_2024_2431_MOESM3_ESM.xlsx Supplemental material (table)

10.5281/zenodo.13685267https://doi.org/10.5281/zenodo.13685267 Zenodo identifier (10.5281/zenodo.13685267, code)

mbexc.de...scopy/https://mbexc.de/visualizing-proteins-by-expansion-microscopy/ Website entry (mbexc.de)

Linked Antibodies

PID	Type	Antigen symbol	Antibody Registry ID	Name
primary-0111	primary	TUBA4A	AB_477583	Anti-α-Tubulin antibody
primary-0124	primary	SNCA/SNCB	AB_887857	Anti-alpha/beta-Synuclein antibody
primary-0149	primary	TUBA1A	AB_887860	Anti-alpha-Tubulin antibody
primary-0179	primary	TUBA4A	AB_887862	Anti-alpha-Tubulin antibody
primary-0255	primary	TUBA4A	AB_2210057	Anti-alpha Tubulin antibody
primary-0636	primary	BSN	AB_11181058	Bassoon monoclonal antibody
primary-1726	primary	SNCA/SNCB	AB_2619811	alpha Synuclein antibody
secondary-1241	secondary		AB_2893229	STAR 635P, goat anti-rabbit IgG
secondary-1713	secondary		AB_2893232	goat anti-mouse IgG STAR 635P

Technical Support: menoci@medizin.uni-goettingen.de

Department of Medical Informatics
University Medical Center Göttingen
Imprint

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External Resources

Linked Antibodies

Technical support

Links

Spokesperson

Prof. Dr. Tobias Moser

Spokesperson of Cluster of Excellence