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Publication

Property	Value
Working Groups	RG Rizzoli, RG Simeth
Subproject	None
Open Access	Yes
Publication Type	Journal Article
Peer Reviewed	Yes
PMID	39384781
DOI	10.1038/s41467-024-53030-w
Publication Year	2024
Title	NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals
Journal	Nature Communications
ISSN	2041-1723
eISSN	2041-1723
URL	http://dx.doi.org/10.1038/s41467-024-53030-w
Issue	1
Volume	15
Journal Abbreviation	Nat Commun
Authors	Mougios N, Cotroneo ER, Imse N, Setzke J, Rizzoli SO, Simeth NA, Tsukanov R, Opazo F
First Author	Mougios N
Last Author	Opazo F

External Resources

nature.c...-w.pdfhttps://www.nature.com/articles/s41467-024-53030-w.pdf Article fulltext

9534https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9534 NCBI taxonomy (African green monkey, Chlorocebus aethiops)

9606https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 NCBI taxonomy (human, Homo sapiens)

10116https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 NCBI taxonomy (rat, Rattus norvegicus)

0009-0007-5712-7175https://orcid.org/0009-0007-5712-7175 ORCID identifier (Nikolaos Mougios

0009-0000-5614-4378https://orcid.org/0009-0000-5614-4378 ORCID identifier (Jonas Setzke)

0000-0002-1667-7839https://orcid.org/0000-0002-1667-7839 ORCID identifier (Silvio-Olivier Rizzoli)

0000-0001-8130-883Xhttps://orcid.org/0000-0001-8130-883X ORCID identifier (Nadja Anita Simeth)

0000-0002-3967-1755https://orcid.org/0000-0002-3967-1755 ORCID identifier (Roman Tsukanov)

0000-0002-4968-9713https://orcid.org/0000-0002-4968-9713 ORCID identifier (Felipe Opazo)

297https://sfb1286.uni-goettingen.de/literature/publications/297 Published Data Registry entry (CRC 1286)

924https://prod.mbexc.uni-goettingen.de/literature/publications/924 Published Data Registry entry (MBExC, preprint)

1/520https://mbexc.uni-goettingen.de/archive/1/520 Research Data Archive (supplemental material)

05xy1nn52https://ror.org/05xy1nn52 ROR identifier (05xy1nn52, Multiscale Bioimaging)

021ft0n22https://ror.org/021ft0n22 ROR identifier (021ft0n22, University Medical Center Göttingen)

01y9bpm73https://ror.org/01y9bpm73 ROR identifier (01y9bpm73, University of Göttingen)

AB_399853https://scicrunch.org/resolver/AB_399853 SciCrunch identifier (RRID:AB_399853)

AB_628381https://scicrunch.org/resolver/AB_628381 SciCrunch identifier (RRID:AB_628381)

AB_628437https://scicrunch.org/resolver/AB_628437 SciCrunch identifier (RRID:AB_628437)

AB_779033https://scicrunch.org/resolver/AB_779033 SciCrunch identifier (RRID:AB_779033)

AB_887717https://scicrunch.org/resolver/AB_887717 SciCrunch identifier (RRID:AB_887717)

AB_887730https://scicrunch.org/resolver/AB_887730 SciCrunch identifier (RRID:AB_887730)

AB_887770https://scicrunch.org/resolver/AB_887770 SciCrunch identifier (RRID:AB_887770)

AB_887792https://scicrunch.org/resolver/AB_887792 SciCrunch identifier (RRID:AB_887792)

AB_887811https://scicrunch.org/resolver/AB_887811 SciCrunch identifier (RRID:AB_887811)

AB_887832https://scicrunch.org/resolver/AB_887832 SciCrunch identifier (RRID:AB_887832)

AB_887859https://scicrunch.org/resolver/AB_887859 SciCrunch identifier (RRID:AB_887859)

AB_1080447https://scicrunch.org/resolver/AB_1080447 SciCrunch identifier (RRID:AB_1080447)

AB_1849446https://scicrunch.org/resolver/AB_1849446 SciCrunch identifier (RRID:AB_1849446)

AB_1857362https://scicrunch.org/resolver/AB_1857362 SciCrunch identifier (RRID:AB_1857362)

AB_2083165https://scicrunch.org/resolver/AB_2083165 SciCrunch identifier (RRID:AB_2083165)

AB_2147096https://scicrunch.org/resolver/AB_2147096 SciCrunch identifier (RRID:AB_2147096)

AB_2545045https://scicrunch.org/resolver/AB_2545045 SciCrunch identifier (RRID:AB_2545045)

AB_2619773https://scicrunch.org/resolver/AB_2619773 SciCrunch identifier (RRID:AB_2619773)

AB_2619811https://scicrunch.org/resolver/AB_2619811 SciCrunch identifier (RRID:AB_2619811)

AB_2619813https://scicrunch.org/resolver/AB_2619813 SciCrunch identifier (RRID:AB_2619813)

AB_3075906https://scicrunch.org/resolver/AB_3075906 SciCrunch identifier (RRID:AB_3075906)

AB_3075980https://scicrunch.org/resolver/AB_3075980 SciCrunch identifier (RRID:AB_3075980)

AB_3075982https://scicrunch.org/resolver/AB_3075982 SciCrunch identifier (RRID:AB_3075982)

AB_3075983https://scicrunch.org/resolver/AB_3075983 SciCrunch identifier (RRID:AB_3075983)

AB_3075986https://scicrunch.org/resolver/AB_3075986 SciCrunch identifier (RRID:AB_3075986)

AB_3076003https://scicrunch.org/resolver/AB_3076003 SciCrunch identifier (RRID:AB_3076003)

AB_3076005https://scicrunch.org/resolver/AB_3076005 SciCrunch identifier (RRID:AB_3076005)

AB_3076025https://scicrunch.org/resolver/AB_3076025 SciCrunch identifier (RRID:AB_3076025)

AB_3076046https://scicrunch.org/resolver/AB_3076046 SciCrunch identifier (RRID:AB_3076046)

AB_3076057https://scicrunch.org/resolver/AB_3076057 SciCrunch identifier (RRID:AB_3076057)

AB_3076106https://scicrunch.org/resolver/AB_3076106 SciCrunch identifier (RRID:AB_3076106)

AB_3076117https://scicrunch.org/resolver/AB_3076117 SciCrunch identifier (RRID:AB_3076117)

AB_3076136https://scicrunch.org/resolver/AB_3076136 SciCrunch identifier (RRID:AB_3076136)

AB_10672335https://scicrunch.org/resolver/AB_10672335 SciCrunch identifier (RRID:AB_10672335)

AB_11042616https://scicrunch.org/resolver/AB_11042616 SciCrunch identifier (RRID:AB_11042616)

AB_11064600https://scicrunch.org/resolver/AB_11064600 SciCrunch identifier (RRID:AB_11064600)

AB_11181058https://scicrunch.org/resolver/AB_11181058 SciCrunch identifier (RRID:AB_11181058)

CVCL_0224https://scicrunch.org/resolver/CVCL_0224 SciCrunch identifier (RRID:CVCL_0224, COS-7)

CVCL_0042https://scicrunch.org/resolver/CVCL_0042 SciCrunch identifier (RRID:CVCL_0042, U2OS)

RGD_13508588https://scicrunch.org/resolver/RGD_13508588 SciCrunch identifier (RRID:RGD_13508588, WI)

SCR_002285https://scicrunch.org/resolver/SCR_002285 SciCrunch identifier (RRID:SCR_002285, Fiji)

SCR_002798https://scicrunch.org/resolver/SCR_002798 SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)

SCR_003070https://scicrunch.org/resolver/SCR_003070 SciCrunch identifier (RRID:SCR_003070, ImageJ)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-024-53030-w/MediaObjects/41467_2024_53030_MOESM1_ESM.pdf Supplemental material

goeseek.gwdguser.de/studies/205https://goeseek.gwdguser.de/studies/205 Supplemental material (goeseek study)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-024-53030-w/MediaObjects/41467_2024_53030_MOESM3_ESM.pdf Supplemental material (peer review)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-024-53030-w/MediaObjects/41467_2024_53030_MOESM2_ESM.pdf Supplemental material (reporting summary)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-024-53030-w/MediaObjects/41467_2024_53030_MOESM4_ESM.xlsx Supplemental material (source data)

mbexc.de...gnals/https://mbexc.de/nanoplex-a-universal-strategy-for-fluorescence-microscopy-multiplexing-using-nanobodies-with-erasable-signals/ Website entry (mbexc.de)

Linked Antibodies

PID	Type	Antigen symbol	Antibody Registry ID	Name
primary-0130	primary	DOC2A/DOC2B	AB_11064600	Anti-Doc 2a/b antibody
primary-0151	primary	VAMP2	AB_887811	Anti-Synaptobrevin 2 antibody
primary-0257	primary	CLTC	AB_2083165	Anti-Clathrin heavy chain antibody
primary-0335	primary	SYT1	AB_887832	Anti-Synaptotagmin 1 antibody
primary-0553	primary	HOMER1	AB_887730	Anti-Homer 1 antibody
primary-0636	primary	BSN	AB_11181058	Bassoon monoclonal antibody
primary-0691	primary	SPTBN2	AB_399853	Purified Mouse Anti-β-Spectrin II
primary-1435	primary	Rab3a	AB_887770	Rab3a antibody
primary-1470	primary	Tom20	AB_628381	Tom20 Antikörper (F-10)
primary-1726	primary	SNCA/SNCB	AB_2619811	alpha Synuclein antibody
primary-1734	primary		AB_628437	Vimentin Antibody (V9)
primary-1735	primary		AB_779033	Anti-Paxillin antibody [Y113]
primary-1736	primary	GEPH	AB_887717	Gephyrin antibody
primary-1737	primary	SNAP25	AB_887792	SNAP25 antibody
primary-1738	primary		AB_887859	alpha-Tubulin antibody
primary-1739	primary	UBTF	AB_1080447	Anti-UBTF antibody produced in rabbit
primary-1740	primary	GALNT2	AB_1849446	Anti-GALNT2 antibody produced in rabbit
primary-1741	primary	SON	AB_1857362	Anti-SON antibody produced in rabbit
primary-1742	primary	MAP2	AB_2147096	MAP2 antibody
primary-1743	primary	VGAT	AB_2545045	VGAT Polyclonal Antibody
primary-1744	primary	SYN1	AB_2619773	Synapsin1/2 antibody
primary-1745	primary	NLGN2	AB_2619813	Neuroligin2 antibody
primary-1746	primary	GFP	AB_3075906	FluoTag®-X4 anti-GFP Atto643
primary-1747	primary	ALFA	AB_3075980	FluoTag®-X2 anti-ALFA AZDye568
primary-1748	primary	ALFA	AB_3075982	FluoTag®-X2 anti-ALFA Atto488
primary-1749	primary	ALFA	AB_3075983	FluoTag®-X2 anti-ALFA Atto643
primary-1750	primary	ALFA	AB_3075986	sdAb anti-ALFA unconjugated
primary-1751	primary	VGlut1	AB_3076003	FluoTag®-X2 anti-VGlut1 AZDye568
primary-1752	primary	VGlut1	AB_3076005	FluoTag®-X2 anti-VGlut1 Atto488
primary-1753	primary	Mouse IgG1	AB_3076025	sdAb anti-Mouse IgG1 unconjugated
primary-1754	primary	Rabbit IgG	AB_3076046	sdAb anti-Rabbit IgG unconjugated
primary-1755	primary	Mouse IgG2a/b	AB_3076057	sdAb anti-Mouse IgG2a/b unconjugated
primary-1756	primary	PSD95	AB_3076106	FluoTag®-X2 anti-PSD95 Atto643
primary-1757	primary	GFAP	AB_3076117	FluoTag®-X2 anti-GFAP Atto488
primary-1758	primary	SYT1	AB_3076136	FluoTag®-X2 anti-Syt1cyt (A51) AZDye568
primary-1759	primary	PMP70		Anti-PMP70 antibody
primary-1760	primary	SV2B	AB_11042616	SV2 B antibody