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 External Resources

 48309https://www.addgene.org/48309  Addgene identifier (48309, reused)

 biorxiv....ll.pdfhttps://www.biorxiv.org/content/10.1101/2021.03.17.435767v1.full.pdf  Article fulltext

 gro-2/100377http://resolver.sub.uni-goettingen.de/purl?gro-2/100377  GRO.publications identifier

 0000-0002-1206-0129https://orcid.org/0000-0002-1206-0129  ORCID identifier (Hauke Sven Hillen)

 0000-0001-6035-4255https://orcid.org/0000-0001-6035-4255  ORCID identifier (Katherine Elizabeth Bohnsack)

 0000-0003-1837-5233https://orcid.org/0000-0003-1837-5233  ORCID identifier (Henning Urlaub)

 0000-0001-8904-1543https://orcid.org/0000-0001-8904-1543  ORCID identifier (Ricarda Richter-Dennerlein)

 PXD023502http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD023502  Pride proteome dataset (PXD023502)

 316https://prod.mbexc.uni-goettingen.de/literature/publications/316  Published Data Registry entry (MBExC, final article)

 03e76ya46https://ror.org/03e76ya46  ROR identifier (03e76ya46, Max Planck Institute for Biophysical Chemistry)

 05xy1nn52https://ror.org/05xy1nn52  ROR identifier (05xy1nn52, Multiscale Bioimaging)

 021ft0n22https://ror.org/021ft0n22  ROR identifier (021ft0n22, University Medical Center Göttingen)

 CVCL_0045https://scicrunch.org/resolver/CVCL_0045  SciCrunch identifier (RRID:CVCL_0045, HEK293)

 CVCL_0063https://scicrunch.org/resolver/CVCL_0063  SciCrunch identifier (RRID:CVCL_0063, HEK293T)

 SCR_014222https://scicrunch.org/resolver/SCR_014222  SciCrunch identifier (RRID:SCR_014222, Coot)

 SCR_016501https://scicrunch.org/resolver/SCR_016501  SciCrunch identifier (RRID:SCR_016501, cryoSPARC)

 SCR_014485https://scicrunch.org/resolver/SCR_014485  SciCrunch identifier (RRID:SCR_014485, MaxQuant)

 SCR_014226https://scicrunch.org/resolver/SCR_014226  SciCrunch identifier (RRID:SCR_014226, MolProbity)

 SCR_014224https://scicrunch.org/resolver/SCR_014224  SciCrunch identifier (RRID:SCR_014224, Phenix)

 SCR_003411https://scicrunch.org/resolver/SCR_003411  SciCrunch identifier (RRID:SCR_003411, PRIDE)

 SCR_016274https://scicrunch.org/resolver/SCR_016274  SciCrunch identifier (RRID:SCR_016274, RELION)

 SCR_017293https://scicrunch.org/resolver/SCR_017293  SciCrunch identifier (RRID:SCR_017293, SerialEM)

 SCR_004097https://scicrunch.org/resolver/SCR_004097  SciCrunch identifier (RRID:SCR_004097, UCSF Chimera)

 CVCL_0045https://scicrunch.org/resolver/CVCL_0045  SciCrunch identifier (RRID:CVCL_0045, HEK293)

 SCR_015872https://scicrunch.org/resolver/SCR_015872  SciCrunch identifier (RRID:SCR_015872, UCSF ChimeraX)

 SCR_018071https://scicrunch.org/resolver/SCR_018071  SciCrunch identifier (RRID:SCR_018071, Warp)

 biorxiv....d=truehttps://www.biorxiv.org/content/biorxiv/early/2021/03/18/2021.03.17.435767/DC1/embed/media-1.mp4?download=true  Supplemental material (video)

 biorxiv....d=truehttps://www.biorxiv.org/content/biorxiv/early/2021/03/18/2021.03.17.435767/DC2/embed/media-2.xlsx?download=true  Supplemental material (table)