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 External Resources

 62988https://www.addgene.org/62988  Addgene identifier (62988, reused)

 nature.c...-1.pdfhttps://www.nature.com/articles/s41467-021-27905-1.pdf  Article fulltext

 gro-2/98794http://resolver.sub.uni-goettingen.de/purl?gro-2/98794  GRO.publications identifier

 GSE174448https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE174448  NCBI GEO Dataset (GSE174448)

 GSE185015https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE185015  NCBI GEO Dataset (GSE185015)

 0000-0003-0844-7547https://orcid.org/0000-0003-0844-7547  ORCID identifier (Nicole Kleiber)

 0000-0001-7545-1656https://orcid.org/0000-0001-7545-1656  ORCID identifier (Nicolas Lemus-Diaz)

 0000-0001-8904-1543https://orcid.org/0000-0001-8904-1543  ORCID identifier (Ricarda Richter-Dennerlein)

 0000-0002-2534-7102https://orcid.org/0000-0002-2534-7102  ORCID identifier (Carina Stiller)

 0000-0002-4548-2299https://orcid.org/0000-0002-4548-2299  ORCID identifier (Claudia Höbartner)

 0000-0001-6035-4255https://orcid.org/0000-0001-6035-4255  ORCID identifier (Katherine Elizabeth Bohnsack)

 0000-0001-7063-5456https://orcid.org/0000-0001-7063-5456  ORCID identifier (Markus T. Bohnsack)

 167https://sfb1190.med.uni-goettingen.de/production/literature/publications/167  Published Data Registry entry (CRC 1190)

 1/7https://mbexc.uni-goettingen.de/archive/1/7  Research Data Archive (supplemental material)

 05xy1nn52https://ror.org/05xy1nn52  ROR identifier (05xy1nn52, Multiscale Bioimaging)

 021ft0n22https://ror.org/021ft0n22  ROR identifier (021ft0n22, University Medical Center Göttingen)

 01y9bpm73https://ror.org/01y9bpm73  ROR identifier (01y9bpm73, University of Göttingen)

 00fbnyb24https://ror.org/00fbnyb24  ROR identifier (00fbnyb24, University of Würzburg)

 CVCL_0045https://scicrunch.org/resolver/CVCL_0045  SciCrunch identifier (RRID:CVCL_0045, HEK293)

 CVCL_0063https://scicrunch.org/resolver/CVCL_0063  SciCrunch identifier (RRID:CVCL_0063, HEK293T)

 SCR_001456https://scicrunch.org/resolver/SCR_001456  SciCrunch identifier (RRID:SCR_001456, BD FACSDiva)

 SCR_002205https://scicrunch.org/resolver/SCR_002205  SciCrunch identifier (RRID:SCR_002205, flowCore)

 SCR_002798https://scicrunch.org/resolver/SCR_002798  SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)

 SCR_013715https://scicrunch.org/resolver/SCR_013715  SciCrunch identifier (RRID:SCR_013715, Image Studio)

 static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-27905-1/MediaObjects/41467_2021_27905_MOESM1_ESM.pdf  Supplemental material

 goeseek.gwdguser.de/studies/116https://goeseek.gwdguser.de/studies/116  Supplemental material (goeseek study)

 static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-27905-1/MediaObjects/41467_2021_27905_MOESM2_ESM.pdf  Supplemental material (reporting summary)

 static-c...SM.ziphttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-021-27905-1/MediaObjects/41467_2021_27905_MOESM3_ESM.zip  Supplemental material (source data)

 mbexc.de...ation/https://mbexc.de/the-rna-methyltransferase-mettl8-installs-m3c32-modifications-in-mitochondrial-trnathr-serucn-to-optimise-trna-structure-and-translation/  Website entry (mbexc.de)