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Publication

Property	Value
Working Groups	RG Fernandez-Busnadiego
Subproject	RA2.1
Open Access	Yes
Publication Type	Preprint
Peer Reviewed	No
DOI	10.1101/2022.02.18.481043
Publication Year	2022
Title	The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds
Journal	bioRxiv
ISSN	no ISSN (NLM ID:101680187)
URL	http://dx.doi.org/10.1101/2022.02.18.481043
Journal Abbreviation	bioRxiv
Extra	Final article published in Nature Communications here: doi:10.1038/s41467-023-36058-2
Authors	Saha I, Yuste-Checa P, Silva Padilha MD, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Ulrich Hartl F, Hipp MS
First Author	Saha I
Last Author	Hipp MS

External Resources

12259https://www.addgene.org/12259 Addgene identifier (12259, reused)

12260https://www.addgene.org/12260 Addgene identifier (12260, reused)

23971https://www.addgene.org/23971 Addgene identifier (23971, reused)

23972https://www.addgene.org/23972 Addgene identifier (23972, reused)

23973https://www.addgene.org/23973 Addgene identifier (23973, reused)

23974https://www.addgene.org/23974 Addgene identifier (23974, reused)

100521https://www.addgene.org/100521 Addgene identifier (100521, reused)

biorxiv....ll.pdfhttps://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full.pdf Article fulltext

gro-2/106453http://resolver.sub.uni-goettingen.de/purl?gro-2/106453 GRO.publications identifier

0000-0002-0289-4082https://orcid.org/0000-0002-0289-4082 ORCID identifier (Itika Saha)

0000-0002-1056-3849https://orcid.org/0000-0002-1056-3849 ORCID identifier (Patricia Yuste Checa)

0000-0003-3520-5439https://orcid.org/0000-0003-3520-5439 ORCID identifier (Qiang Guo)

0000-0002-2050-413Xhttps://orcid.org/0000-0002-2050-413X ORCID identifier (Hauke Holthusen)

0000-0001-8964-6120https://orcid.org/0000-0001-8964-6120 ORCID identifier (Victoria Anne Trinkaus)

0000-0003-1052-8485https://orcid.org/0000-0003-1052-8485 ORCID identifier (Irina Dudanova)

0000-0002-8366-7622https://orcid.org/0000-0002-8366-7622 ORCID identifier (Ruben Fernandez-Busnadiego)

0000-0002-7941-135Xhttps://orcid.org/0000-0002-7941-135X ORCID identifier (F. Ulrich Hartl)

0000-0002-0497-3016https://orcid.org/0000-0002-0497-3016 ORCID identifier (Mark S. Hipp)

654https://prod.mbexc.uni-goettingen.de/literature/publications/654 Published Data Registry entry (MBExC, final article)

1/300https://mbexc.uni-goettingen.de/archive/1/300 Research Data Archive (supplemental material)

03zj4c476https://ror.org/03zj4c476 ROR identifier (03zj4c476, Aligning Science Across Parkinson's)

033n9gh91https://ror.org/033n9gh91 ROR identifier (033n9gh91, Carl von Ossietzky Universität Oldenburg)

04py35477https://ror.org/04py35477 ROR identifier (04py35477, Max Planck Institute of Biochemistry)

03ap2av50https://ror.org/03ap2av50 ROR identifier (03ap2av50, Max Planck Institute of Neurobiology)

025z3z560https://ror.org/025z3z560 ROR identifier (025z3z560, Munich Cluster for Systems Neurology)

05xy1nn52https://ror.org/05xy1nn52 ROR identifier (05xy1nn52, Multiscale Bioimaging)

05byvp690https://ror.org/05byvp690 ROR identifier (05byvp690, The University of Texas Southwestern Medical Center)

021ft0n22https://ror.org/021ft0n22 ROR identifier (021ft0n22, University Medical Center Göttingen)

01y9bpm73https://ror.org/01y9bpm73 ROR identifier (01y9bpm73, University of Göttingen)

012p63287https://ror.org/012p63287 ROR identifier (012p63287, University of Groningen)

SCR_007353https://scicrunch.org/resolver/SCR_007353 SciCrunch identifier (RRID:SCR_007353, Advanced 3D Visualization and Volume Modeling)

SCR_002285https://scicrunch.org/resolver/SCR_002285 SciCrunch identifier (RRID:SCR_002285, Fiji)

SCR_008520https://scicrunch.org/resolver/SCR_008520 SciCrunch identifier (RRID:SCR_008520, FlowJo)

SCR_002798https://scicrunch.org/resolver/SCR_002798 SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)

SCR_003070https://scicrunch.org/resolver/SCR_003070 SciCrunch identifier (RRID:SCR_003070, ImageJ)

SCR_003297https://scicrunch.org/resolver/SCR_003297 SciCrunch identifier (RRID:SCR_003297, IMOD)

SCR_014485https://scicrunch.org/resolver/SCR_014485 SciCrunch identifier (RRID:SCR_014485, MaxQuant)

SCR_016499https://scicrunch.org/resolver/SCR_016499 SciCrunch identifier (RRID:SCR_016499, MotionCor 2)

SCR_015753https://scicrunch.org/resolver/SCR_015753 SciCrunch identifier (RRID:SCR_015753, Perseus)

SCR_017293https://scicrunch.org/resolver/SCR_017293 SciCrunch identifier (RRID:SCR_017293, SerialEM)

SCR_013672https://scicrunch.org/resolver/SCR_013672 SciCrunch identifier (RRID:SCR_013672, ZEN Digital Imaging for Light Microscopy)

biorxiv....d=truehttps://www.biorxiv.org/content/biorxiv/early/2022/02/19/2022.02.18.481043/DC1/embed/media-1.pdf?download=true Supplemental material

mbexc.de...seeds/https://mbexc.de/the-aaa-chaperone-vcp-disaggregates-tau-fibrils-and-generates-aggregate-seeds/ Website entry (mbexc.de)

Linked Antibodies

PID	Type	Antigen symbol	Antibody Registry ID	Name
primary-0008	primary	GAPDH	AB_2107445	Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody
primary-0111	primary	TUBA4A	AB_477583	Anti-α-Tubulin antibody
primary-0185	primary	MAPT	AB_223647	Phospho-Tau (Ser202, Thr205) Monoclonal Antibody
primary-0190	primary	UBB	AB_1587578	Anti-Ubiquitin Antibody
primary-0193	primary	VCP	AB_298039	Anti-VCP antibody
primary-0619	primary	ATG7	AB_10831194	Atg7 (D12B11) antibody
primary-0784	primary	GFP	AB_390913	Anti-GFP antibody
primary-0988	primary	VCP	AB_2304347	p97/VCP Antibody
primary-0989	primary	UBB	AB_2895239	Recombinant Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
primary-0990	primary	UBB	AB_628423	Anti-Ubiquitin Antibody (P4D1)
primary-0991	primary	MAPT	AB_11167058	Tau (phospho Ser356) antibody
primary-0992	primary	NPLOC4	AB_1854586	Anti-NPLOC4 antibody
primary-0993	primary	UFD1	AB_10678868	Anti-UFD1L antibody
primary-0994	primary	Ubiquitinylated proteins	AB_612093	Anti-Ubiquitinylated proteins Antibody
primary-0995	primary	MAPT	AB_10980631	Tau Monoclonal Antibody
primary-0996	primary	MAPT	AB_2341273	Anti-Human Tau/Repeat Domain (2B11) Mouse IgG MoAb
primary-0997	primary	MAP1LC3B	AB_796155	Anti-LC3B antibody
primary-0998	primary	ATG5	AB_2062340	Atg5 Antibody
primary-0999	primary	PSMD11	AB_2268979	PSMD11 Polyclonal antibody
secondary-0042	secondary		AB_2534033	Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5
secondary-0376	secondary		AB_258434	Anti-Rabbit IgG (whole molecule)–Peroxidase antibody
secondary-1000	secondary		AB_2534032	Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5
secondary-1001	secondary		AB_2340863	Alexa Fluor® 647 AffiniPure Donkey Anti-Mouse IgG (H+L)
secondary-1002	secondary		AB_2556746	Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488
secondary-1003	secondary		AB_258167	Anti-Mouse IgG (whole molecule)–Peroxidase antibody
secondary-1004	secondary		AB_10956588	IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody
secondary-1005	secondary		AB_621843	IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody