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Publication

Property	Value
Working Groups	RG Fernandez-Busnadiego
Subproject	RA2.1
Open Access	Yes
Publication Type	Journal Article
Peer Reviewed	Yes
PMID	36732333
DOI	10.1038/s41467-023-36058-2
Publication Year	2023
Title	The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system
Journal	Nature Communications
ISSN	2041-1723
eISSN	2041-1723
URL	http://dx.doi.org/10.1038/s41467-023-36058-2
Issue	1
Volume	14
Journal Abbreviation	Nat Commun
Authors	Saha I, Yuste-Checa P, Da Silva Padilha M, Guo Q, Körner R, Holthusen H, Trinkaus VA, Dudanova I, Fernández-Busnadiego R, Baumeister W, Sanders DW, Gautam S, Diamond MI, Hartl FU, Hipp MS
First Author	Saha I
Last Author	Hipp MS

External Resources

12259https://www.addgene.org/12259 Addgene identifier (12259, reused)

12260https://www.addgene.org/12260 Addgene identifier (12260, reused)

23971https://www.addgene.org/23971 Addgene identifier (23971, reused)

23972https://www.addgene.org/23972 Addgene identifier (23972, reused)

23973https://www.addgene.org/23973 Addgene identifier (23973, reused)

23974https://www.addgene.org/23974 Addgene identifier (23974, reused)

100521https://www.addgene.org/100521 Addgene identifier (100521, reused)

188572https://www.addgene.org/188572 Addgene identifier (188572)

188573https://www.addgene.org/188573 Addgene identifier (188573)

nature.c...-2.pdfhttps://www.nature.com/articles/s41467-023-36058-2.pdf Article fulltext

EMD-13739https://www.ebi.ac.uk/pdbe/emdb/EMD-13739 EMBL-EBI EMDB identifier (EMD-13739)

EMD-13740https://www.ebi.ac.uk/pdbe/emdb/EMD-13740 EMBL-EBI EMDB identifier (EMD-13740)

13579https://instruments.goettingen-research-online.de/Landing/Resource?id=13579 GRO.instruments resource identifier (13579, Krios G4 Cryo-TEM)

gro-2/121729http://resolver.sub.uni-goettingen.de/purl?gro-2/121729 GRO.publications identifier

9606https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 NCBI taxonomy (human, Homo sapiens)

0000-0002-1056-3849https://orcid.org/0000-0002-1056-3849 ORCID identifier (Patricia Yuste Checa)

0000-0003-3520-5439https://orcid.org/0000-0003-3520-5439 ORCID identifier (Qiang Guo)

0000-0002-2050-413Xhttps://orcid.org/0000-0002-2050-413X ORCID identifier (Hauke Holthusen)

0000-0001-8964-6120https://orcid.org/0000-0001-8964-6120 ORCID identifier (Victoria Anne Trinkaus)

0000-0003-1052-8485https://orcid.org/0000-0003-1052-8485 ORCID identifier (Irina Dudanova)

0000-0002-8366-7622https://orcid.org/0000-0002-8366-7622 ORCID identifier (Ruben Fernandez-Busnadiego)

0000-0001-8154-8809https://orcid.org/0000-0001-8154-8809 ORCID identifier (Wolfgang Baumeister)

0000-0003-0366-6169https://orcid.org/0000-0003-0366-6169 ORCID identifier (Saurabh Gautam)

0000-0002-8085-7770https://orcid.org/0000-0002-8085-7770 ORCID identifier (Marc I. Diamond)

0000-0002-7941-135Xhttps://orcid.org/0000-0002-7941-135X ORCID identifier (F. Ulrich Hartl)

PXD023400http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD023400 Pride proteome dataset (PXD023400)

449https://prod.mbexc.uni-goettingen.de/literature/publications/449 Published Data Registry entry (MBExC, preprint)

1/122https://mbexc.uni-goettingen.de/archive/1/122 Research Data Archive (supplemental material)

05xy1nn52https://ror.org/05xy1nn52 ROR identifier (05xy1nn52, Multiscale Bioimaging)

021ft0n22https://ror.org/021ft0n22 ROR identifier (021ft0n22, University Medical Center Göttingen)

AB_223647https://scicrunch.org/resolver/AB_223647 SciCrunch identifier (RRID:AB_223647)

AB_258167https://scicrunch.org/resolver/AB_258167 SciCrunch identifier (RRID:AB_258167)

AB_258434https://scicrunch.org/resolver/AB_258434 SciCrunch identifier (RRID:AB_258434)

AB_298039https://scicrunch.org/resolver/AB_298039 SciCrunch identifier (RRID:AB_298039)

AB_390913https://scicrunch.org/resolver/AB_390913 SciCrunch identifier (RRID:AB_390913)

AB_477583https://scicrunch.org/resolver/AB_477583 SciCrunch identifier (RRID:AB_477583)

AB_527461https://scicrunch.org/resolver/AB_527461 SciCrunch identifier (RRID:AB_527461)

AB_612093https://scicrunch.org/resolver/AB_612093 SciCrunch identifier (RRID:AB_612093)

AB_621843https://scicrunch.org/resolver/AB_621843 SciCrunch identifier (RRID:AB_621843)

AB_628423https://scicrunch.org/resolver/AB_628423 SciCrunch identifier (RRID:AB_628423)

AB_796155https://scicrunch.org/resolver/AB_796155 SciCrunch identifier (RRID:AB_796155)

AB_1587578https://scicrunch.org/resolver/AB_1587578 SciCrunch identifier (RRID:AB_1587578)

AB_1587580https://scicrunch.org/resolver/AB_1587580 SciCrunch identifier (RRID:AB_1587580)

AB_1854586https://scicrunch.org/resolver/AB_1854586 SciCrunch identifier (RRID:AB_1854586)

AB_2062340https://scicrunch.org/resolver/AB_2062340 SciCrunch identifier (RRID:AB_2062340)

AB_2107445https://scicrunch.org/resolver/AB_2107445 SciCrunch identifier (RRID:AB_2107445)

AB_2266850https://scicrunch.org/resolver/AB_2266850 SciCrunch identifier (RRID:AB_2266850)

AB_2268979https://scicrunch.org/resolver/AB_2268979 SciCrunch identifier (RRID:AB_2268979)

AB_2340863https://scicrunch.org/resolver/AB_2340863 SciCrunch identifier (RRID:AB_2340863)

AB_2341273https://scicrunch.org/resolver/AB_2341273 SciCrunch identifier (RRID:AB_2341273)

AB_2534032https://scicrunch.org/resolver/AB_2534032 SciCrunch identifier (RRID:AB_2534032)

AB_2534033https://scicrunch.org/resolver/AB_2534033 SciCrunch identifier (RRID:AB_2534033)

AB_2556746https://scicrunch.org/resolver/AB_2556746 SciCrunch identifier (RRID:AB_2556746)

AB_10678868https://scicrunch.org/resolver/AB_10678868 SciCrunch identifier (RRID:AB_10678868)

AB_10831194https://scicrunch.org/resolver/AB_10831194 SciCrunch identifier (RRID:AB_10831194)

AB_10956588https://scicrunch.org/resolver/AB_10956588 SciCrunch identifier (RRID:AB_10956588)

AB_10980631https://scicrunch.org/resolver/AB_10980631 SciCrunch identifier (RRID:AB_10980631)

AB_11167058https://scicrunch.org/resolver/AB_11167058 SciCrunch identifier (RRID:AB_11167058)

CVCL_0045https://scicrunch.org/resolver/CVCL_0045 SciCrunch identifier (RRID:CVCL_0045, HEK293)

CVCL_0063https://scicrunch.org/resolver/CVCL_0063 SciCrunch identifier (RRID:CVCL_0063, HEK293T)

CVCL_C1CRhttps://scicrunch.org/resolver/CVCL_C1CR SciCrunch identifier (RRID:CVCL_C1CR, HEK293T Tet-off FLTau)

CVCL_C1CShttps://scicrunch.org/resolver/CVCL_C1CS SciCrunch identifier (RRID:CVCL_C1CS, HEK293T Tet-off TauRD)

SCR_007353https://scicrunch.org/resolver/SCR_007353 SciCrunch identifier (RRID:SCR_007353, Advanced 3D Visualization and Volume Modeling)

SCR_002285https://scicrunch.org/resolver/SCR_002285 SciCrunch identifier (RRID:SCR_002285, Fiji)

SCR_008520https://scicrunch.org/resolver/SCR_008520 SciCrunch identifier (RRID:SCR_008520, FlowJo)

SCR_002798https://scicrunch.org/resolver/SCR_002798 SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)

SCR_003297https://scicrunch.org/resolver/SCR_003297 SciCrunch identifier (RRID:SCR_003297, IMOD)

SCR_014485https://scicrunch.org/resolver/SCR_014485 SciCrunch identifier (RRID:SCR_014485, MaxQuant)

SCR_016137https://scicrunch.org/resolver/SCR_016137 SciCrunch identifier (RRID:SCR_016137, Microsoft Excel)

SCR_016499https://scicrunch.org/resolver/SCR_016499 SciCrunch identifier (RRID:SCR_016499, MotionCor2)

SCR_014212https://scicrunch.org/resolver/SCR_014212 SciCrunch identifier (RRID:SCR_014212, Origin)

SCR_015753https://scicrunch.org/resolver/SCR_015753 SciCrunch identifier (RRID:SCR_015753, Perseus)

SCR_017293https://scicrunch.org/resolver/SCR_017293 SciCrunch identifier (RRID:SCR_017293, SerialEM)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-36058-2/MediaObjects/41467_2023_36058_MOESM1_ESM.pdf Supplemental material

goeseek.gwdguser.de/studies/51https://goeseek.gwdguser.de/studies/51 Supplemental material (goeseek study)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-36058-2/MediaObjects/41467_2023_36058_MOESM2_ESM.pdf Supplemental material (peer review)

dx.doi.o...gmk/v1https://dx.doi.org/10.17504/protocols.io.6qpvr4xn3gmk/v1 Supplemental material (protocols.io)

dx.doi.o...g25/v1https://dx.doi.org/10.17504/protocols.io.kqdg39eo1g25/v1 Supplemental material (protocols.io)

dx.doi.o...lr2/v1https://dx.doi.org/10.17504/protocols.io.ewov1ojwklr2/v1 Supplemental material (protocols.io)

dx.doi.o...g3e/v1https://dx.doi.org/10.17504/protocols.io.x54v9den4g3e/v1 Supplemental material (protocols.io)

dx.doi.o...l5b/v1https://dx.doi.org/10.17504/protocols.io.n92ldpbx8l5b/v1 Supplemental material (protocols.io)

dx.doi.o...lo5/v1https://dx.doi.org/10.17504/protocols.io.3byl4je4rlo5/v1 Supplemental material (protocols.io)

dx.doi.o...vk5/v1https://dx.doi.org/10.17504/protocols.io.36wgqjrzyvk5/v1 Supplemental material (protocols.io)

dx.doi.o...g3e/v1https://dx.doi.org/10.17504/protocols.io.x54v9jnrzg3e/v1 Supplemental material (protocols.io)

dx.doi.o...g1y/v1https://dx.doi.org/10.17504/protocols.io.4r3l27mjpg1y/v1 Supplemental material (protocols.io)

static-c...SM.pdfhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-36058-2/MediaObjects/41467_2023_36058_MOESM3_ESM.pdf Supplemental material (reporting summary)

static-c...M.xlsxhttps://static-content.springer.com/esm/art%3A10.1038%2Fs41467-023-36058-2/MediaObjects/41467_2023_36058_MOESM4_ESM.xlsx Supplemental material (source data)

UP000005640https://www.uniprot.org/proteomes/UP000005640 UniProt Proteome identifier (UP000005640)

mbexc.de...ystem/https://mbexc.de/the-aaa-chaperone-vcp-disaggregates-tau-fibrils-and-generates-aggregate-seeds-in-a-cellular-system/ Website entry (mbexc.de)

Linked Antibodies

PID	Type	Antigen symbol	Antibody Registry ID	Name
primary-0008	primary	GAPDH	AB_2107445	Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody
primary-0111	primary	TUBA4A	AB_477583	Anti-α-Tubulin antibody
primary-0185	primary	MAPT	AB_223647	Phospho-Tau (Ser202, Thr205) Monoclonal Antibody
primary-0190	primary	UBB	AB_1587578	Anti-Ubiquitin Antibody
primary-0191	primary	UBB	AB_1587580	Anti-Ubiquitin Antibody
primary-0193	primary	VCP	AB_298039	Anti-VCP antibody
primary-0619	primary	ATG7	AB_10831194	Atg7 (D12B11) antibody
primary-0784	primary	GFP	AB_390913	Anti-GFP antibody
primary-0990	primary	UBB	AB_628423	Anti-Ubiquitin Antibody (P4D1)
primary-0991	primary	MAPT	AB_11167058	Tau (phospho Ser356) antibody
primary-0992	primary	NPLOC4	AB_1854586	Anti-NPLOC4 antibody
primary-0993	primary	UFD1	AB_10678868	Anti-UFD1L antibody
primary-0994	primary	Ubiquitinylated proteins	AB_612093	Anti-Ubiquitinylated proteins Antibody
primary-0995	primary	MAPT	AB_10980631	Tau Monoclonal Antibody
primary-0996	primary	MAPT	AB_2341273	Anti-Human Tau/Repeat Domain (2B11) Mouse IgG MoAb
primary-0997	primary	MAP1LC3B	AB_796155	Anti-LC3B antibody
primary-0998	primary	ATG5	AB_2062340	Atg5 Antibody
primary-0999	primary	PSMD11	AB_2268979	PSMD11 Polyclonal antibody
primary-1199	primary	VCP	AB_527461	p97/VCP Antibody
primary-1200	primary	MYC	AB_2266850	c-Myc antibody (9E 10)
secondary-0042	secondary		AB_2534033	Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5
secondary-0376	secondary		AB_258434	Anti-Rabbit IgG (whole molecule)–Peroxidase antibody
secondary-1000	secondary		AB_2534032	Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine5
secondary-1001	secondary		AB_2340863	Alexa Fluor® 647 AffiniPure Donkey Anti-Mouse IgG (H+L)
secondary-1002	secondary		AB_2556746	Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 488
secondary-1003	secondary		AB_258167	Anti-Mouse IgG (whole molecule)–Peroxidase antibody
secondary-1004	secondary		AB_10956588	IRDye® 680RD Goat anti-Mouse IgG Secondary Antibody
secondary-1005	secondary		AB_621843	IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody